The court made its ruling on June 7 for breaches of the Stock Act 1915, and all parties were charged with introducing stock into Queensland without a health certificate.

The matter was heard over two court hearings in which two parties were fined $50,000 each and the remaining three parties were collectively fined $14,000 for the offences.

This is the biggest collective fine for this offence in Queensland.

Three parties were also charged with introducing a prescribed species into Queensland relating to cattle from a Bovine Johne's Disease infected herd.

The breaches related to two different consignments of cattle totalling 198 beasts which were moved from NSW into Queensland in March 2009.

The first consignment did not have the necessary health certificate, and 36 beasts were found to have come from an infected Bovine Johne's Disease herd.

In the second consignment, all 100 beasts were also found to have come from an infected Bovine Johne's Disease herd.

Biosecurity Queensland investigator Craig Elliott said any cattle that pose a biosecurity risk, including those suspected of carrying Bovine Johne's disease will be quarantined.

"Any person involved in the movement of livestock into Queensland, including the owners of the cattle and anyone else in the supply chain such as transport companies can be held responsible for illegal entry," he said.

"This case has shown how seriously we consider the illegal entry of livestock into Queensland, and how important it is to protect our state's biosecurity."

More information about the entry requirements for cattle to be brought into Queensland can be found at www.biosecurity.qld.gov.au